The Antibiotic Resistance-Related Indicators In Campylobacter Species Isolated From Water And Retail Fresh Milk Samples In Southern Iraq
Keywords:
Resistance-Related, Campylobacter, Water, Fresh MilkAbstract
Campylobacter species are prominent bacteria associated with human gastrointestinal diseases and are primarily present in the faeces of domestic animals, sewage discharge and agricultural runoff. These viruses have been linked to disease outbreaks resulting from the intake of contaminated water and milk in many regions of the world. However, there is a lack of detailed reports on this issue in the Misan Governorate. Therefore, this study assessed the prevalence, pathogenicity and antibiotic resistance markers of Campylobacter species isolated from water and milk samples. A combined total of 70 raw milk samples and 57 water samples were gathered. These samples were subjected to enrichment in Bolton broth and then incubated for 48 h at 42 °C in a 10% CO2 environment with limited oxygen. Subsequently, the cultures that had been enriched were subjected to additional processing and purification. Campylobacter colonies that were likely to be present were separated and verified by PCR using specialised primers designed to identify the Campylobacter genus, specific species and genes linked with virulence. The antimicrobial resistance profiles of the isolates were examined using the disc diffusion method against a panel of 12 antibiotics. The presence of important genotypic resistance genes was assessed by PCR. A total of 309 presumptive Campylobacter isolates were collected, of which 180 were confirmed to be from the Campylobacter genusReferences
1. Abdul-Rahman, S. M. (2019). Bacteriological Profile, Molecular Detection and
Antimicrobial Susceptibility Test among Pre-term Babies in Erbil city, Iraq (Doctoral
dissertation, Salahaddin University-Erbil).
2. Admasie, A., Eshetu, A., Tessema, T. S., Vipham, J., Kovac, J., & Zewdu, A. (2023).
Prevalence of Campylobacter species and associated risk factors for contamination of dairy
products collected in a dry season from major milk sheds in Ethiopia. Food
Microbiology, 109, 104145.
3. Adzitey, F., Rusul, G., Huda, N., Cogan, T., & Corry, J. (2012). Prevalence, antibiotic
resistance and RAPD typing of Campylobacter species isolated from ducks, their rearing and
processing environments in Penang, Malaysia. International journal of food
microbiology, 154(3), 197-205.
4. Ali, J. A., & Bunyan, I. A. (2021). Pathogenic Virus And Bacteria Associated With Diarrhea
Among Children In Hilla Province, Iraq. Biochemical & Cellular Archives, 21(2).
5. Almashhadany, D. A. (2021). Isolation, biotyping and antimicrobial susceptibility of
Campylobacter isolates from raw milk in Erbil city, Iraq. Italian Journal of Food
Safety, 10(1).
6. Al-Mawla, S., J Al-Dalla Ali, F., & M Al-Ani, M. (2008). The role of campylobacter species
in diarrhea among children under five years of age in Ramadi city, west of Iraq. Al-Anbar
Medical Journal, 6(1), 76-87.
7. Amarasiri, M., Sano, D., & Suzuki, S. (2020). Understanding human health risks caused by
antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water
environments: Current knowledge and questions to be answered. Critical Reviews in
Environmental Science and Technology, 50(19), 2016-2059.
8. Ammar, A. M., El-Naenaeey, E. S. Y., Abd El-Hamid, M. I., El-Gedawy, A. A., & Elmalt, R.
M. (2021). Campylobacter as a major foodborne pathogen: A review of its characteristics,
pathogenesis, antimicrobial resistance and control. Journal of microbiology, biotechnology
and food sciences, 10(4), 609-619.
9. Andrzejewska, M., Szczepańska, B., Śpica, D., & Klawe, J. J. (2019). Prevalence, virulence,
and antimicrobial resistance of Campylobacter spp. in raw milk, beef, and pork meat in
Northern Poland. Foods, 8(9), 420.
10. Andrzejewska, M., Szczepańska, B., Śpica, D., & Klawe, J. J. (2019). Prevalence, virulence,
and antimicrobial resistance of Campylobacter spp. in raw milk, beef, and pork meat in
Northern Poland. Foods, 8(9), 420.
11. Aprea, G., & Mullan, W. M. A. (2022). Raw milk: benefits and hazards. In Dairy Foods (pp.
19-46). Woodhead Publishing.
12. Bianchini, V., Borella, L., Benedetti, V., Parisi, A., Miccolupo, A., Santoro, E., ... & Luini,
M. (2014). Prevalence in bulk tank milk and epidemiology of Campylobacter jejuni in dairy
herds in Northern Italy. Applied and environmental microbiology, 80(6), 1832-1837.
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